The detection limits of this kit meet all of the EU MRLs for fluoroquinolones in meat
- Rapid strip test method – less than 20 minutes
- Broad-spectrum antibody that requires no heating step
- High sensitivity
- High reproducibility
- Interpretation of results can be done by eye or using the QuickSTAR™ Strip Reader
Qualitative, Rapid Lateral Flow Test for the Detection of Fluoroquinolone Residues in Meat
The AuroFlow™ Fluoroquinolone Strip Test Kit for Meat is a qualitative and rapid lateral flow assay designed to detect fluoroquinolone antibiotic residues in meat samples such as chicken and beef. This state-of-the-art test uses a broad-spectrum antibody capable of specifically detecting a range of fluoroquinolone residues, and is designed for rapid field use or reference lab settings and requires no expensive lab equipment such as heaters and centrifuges.
The assay uses a competitive colloidal gold based format. The extracted meat sample (200 μL) is added to a clear plastic reaction vessel, and used to resuspend the lyophilized reagents to a uniform pink color in the bottom of the microtiter wells. The sample is incubated briefly (3 min) to allow the fluoroquinolone antibody on the gold particles to engage with any fluoroquinolone antibiotic residues present. The test strip is then inserted into the sample well with the arrows pointing downward initiating capillary flow up the strip. Any gold particles that are not complexed with antibiotics present in the sample will bind to the fluoroquinolone antibiotic imprinted at the Test line (T-line), forming a signal (red line) at that position. If the fluoroquinolone antibody on the gold particle has engaged with antibiotic present in the sample, the gold particle will flow past the T-line and reach the Control line (C-line). For visual interpretation of the test results, T-line signal intensity that is stronger than the signal at the C-line indicates a negative result. Signal at the T-line which is equal or less intense compared to the C-line indicates the presence of fluoroquinolone antibiotics in the meat. The greater the reduction in signal intensity at the T-line, the greater the concentration of fluoroquinolone antibiotic residues present in the sample.
Fluoroquinolones are a group of antibiotics that can inhibit the activity of bacterial DNA gyrase. The fluoroquinolone group mainly includes enrofloxacin and its active metabolite ciprofloxacin, danofloxacin, difloxacin, norfloxacin, pipemidic acid, ofloxacin, benofloxacin and others. One of widely used fluoroquinolones is enrofloxacin, which was the first fluoroquinolone antimicrobial to be used in veterinary infections by E. coli, Salmonella, Pasteurella, Mycoplasma and Hemophilus species. The MRLs for the sum of enrofloxacin and its active metabolite ciprofloxacin have been stated at 100 ppb for muscle tissue and fat.
This kit is manufactured to the international quality standard ISO 9001:2008.
The AuroFlow™ Fluoroquinolone Strip Test Kit for Meat has the capacity for 32 (Cat # 1102-03) determinations. The shelf life is 24 months when the kit is properly stored.