- One-step competitive ELISA
- Sensitive Crystal Violet detection – 0.045 ppb for fish and shrimp samples
- High recovery, cost-effective extraction methods for fish/shrimp or water samples
- Saves money - No expensive instrumentation required
- Easy, high recovery sample preparation
- 96-well high throughput quantitative detection method
- Prepared samples can be directly used for liquid chromatography confirmation
A One-step ELISA for the Screening of Crystal Violet/LCV in Fish, Shrimp, Fish Oil and Water
The patent-pending MaxSignal® Crystal Violet/LCV ELISA Test Kit is designed for routine screening for Crystal Violet/Leucocrystal Violet in fish, shrimp, fish and shrimp feed, fish oil and water samples of fish farms or fishponds.
The MaxSignal Crystal Violet/LCV ELISA Test Kit is a one-step, competitive, colorimetric ELISA assay. The Crystal Violet (CV) antibody has been coated in the plate wells. During the analysis, sample is added along with the CV-horseradish peroxidase (CV-HRP) conjugate. If the CV residue is present in the sample, it will compete for the CV antibody, thereby preventing the CV-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the CV residue concentration in the sample.
Crystal Violet has been widely used all over the world in the fish farming industry as a fungicide, ectoparasiticide and disinfectant. However, it has been shown recently that Crystal Violet is highly cytotoxic to mammalian cells and has been implicated in the induction of thyroid and liver tumors. This has led to a prohibition of Crystal Violet for the treatment of fish and shrimp used for food production.
Publications that Reference Using the MaxSignal Crystal Violet ELISA
Contia, G. O., Chiara Copata, C., Wangb, Z., D’Agatia, P., Cristaldia, A. and Ferrantea, M. (2014) Determination of illegal antimicrobials in aquaculture feed and fish: An ELISA study. Food Control. Doi: 10.1016/j.foodcont.2014.10.050
This kit is manufactured to the international quality standard ISO 9001:2008.
PLATE: 1 x 96 well (Removable 8 wells x 12 strips)
MEASUREMENT: microtiter plate reader (450 nm)
TESTS PER KIT: The kit has the capacity for 96 determinations or testing of 42 samples in duplicate
STANDARDS: 0 / 0.02 / 0.067 / 0.2 / 0.67 / 2
SHELF LIFE & STORAGE: 12 months when properly stored.