Rapid extraction methods take less than 40 minutes
- High recovery rates
- High sensitivity - 0.5 ppb
- Low detection limit of 2.5 ppb for fish and shrimp samples
- Quick ELISA assay requires less than 2 hours regardless of number of samples
- High reproducibility
ELISA for the Screening of Erythromycin in Meat, Fish and Shrimp Samples
The MaxSignal® Erythromycin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of erythromycin in meat, fish and shrimp samples.
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Erythromycin is a macrolide antibiotic, which is used to treat bacterial and mycoplasma infections. It also can be used as feed additives to improve feed transformation efficiency and increase body weight.
This kit is manufactured to the international quality standard ISO 9001:2008.
The MaxSignal® Erythromycin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C. The shelf life is at least 12 months when the kit is properly stored.