Sensitive Gentamicin detection – Detection limit of 5.0 ppb for meat and milk; 10.0 ppb for egg, serum and urine; 12.5 ppb for feed and honey
- Cost-effective extraction methods with high recovery rates
- Faster results – Less than 2 hours from sample to results
- Saves money - No expensive instrumentation required
- Easy, high recovery sample preparation
ELISA for the Analysis of Gentamicin Residues in Food
MaxSignal® Gentamicin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Gentamicin in egg, feed, honey, meat, liver, kidney, milk, serum and urine.
Gentamicin is an aminoglycoside antibiotic that is hydrosoluble, polar heat stable and slightly basic. Gentamicin’s antibacterial activity is due to its ability to bind to the 30S subunit of the bacterial ribosome where it inhibits protein synthesis. All the aminoglycosides are potentially toxic compounds causing significant damage in vestibular and auditory functions in human and animals. Nevertheless, they are used in practice because of their antibacterial and antifungal activities. These compounds have been found to be useful for the treatment of serious infections due to Gram negative microorganisms.
The European Commission established a gentamicin Maximum Residue Limit (MRL) of 50 ppb for muscle and fat, 200 ppb for liver, 750 ppb for kidney and 100 ppb for milk.
Publications that Reference Using the MaxSignal Gentamicin ELISA
Contia, G. O., Chiara Copata, C., Wangb, Z., D’Agatia, P., Cristaldia, A. and Ferrantea, M. (2014) Determination of illegal antimicrobials in aquaculture feed and fish: An ELISA study. Food Control. Doi: 10.1016/j.foodcont.2014.10.050.
Hunter, B. G., et. al. (2015) Morphine Synovial Fluid Concentrations after Intravenous Regional Limb Perfusion in Standing Horses. Veterinary Surgery. doi: 10.1111/j.1532-950X.2014.12314.x.
Kuang, J., Guo, J. L. and Messersmith, P. B. (2014), High Ionic Strength Formation of DOPA-Melanin Coating for Loading and Release of Cationic Antimicrobial Compounds. Advanced Materials Interfaces. doi: 10.1002/admi.201400145.
This kit is manufactured to the international quality standard ISO 9001:2008.
PLATE: 1 x 96 well (Removable 8 wells x 12 strips)
MEASUREMENT: microtiter plate reader (450 nm)
TESTS PER KIT: The kit has the capacity for 96 determinations or testing of 42 samples in duplicate
STANDARDS: 0 / 0.5 / 1.0 / 2.5 / 5.0 / 15 ppb
INCUBATION TIME: 75 minutes
SHELF LIFE & STORAGE: 12 months when properly stored