MaxSignal® Nitrofurantoin (AHD) ELISA Kit

 

Catalog# Product Name Quantity US List Price
1070-02F MaxSignal® Nitrofurantoin (AHD) ELISA Kit
1 x 96 wells
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These kits are available for purchase by email, fax or phone only. Please email your order to sales@biooscientific.com, fax it to 1-512-707-8122 or call 512-707-8993.

 

 

  • Offers a rapid and cost-effective extraction methods for various samples offering recovery rates
  • High sensitivity - 0.025 ppb
  • Best standard range available - 0.025 - 6.4 ppb
  • Low detection limit - 0.05 ppb
  • Offers a single extraction protocol to for the analysis of AHD, SEM, AMOZ and AOZ
  • A quick ELISA assay requiring less than 1.5 hours regardless of number of samples
  • High reproducibility

 

 

 

Sensitive ELISA for the Detection of AHD in Food Samples

The MaxSignal® Nitrofurantoin (AHD) ELISA Kit provides a competitive enzyme immunoassay for the quantitative analysis of nitrofurantoin in feed, fish, shrimp, meat, eggs, honey, milk, serum and urine. The new version of this kit allows for the accurate quantitation of AHD at a broader range than any of the other commercially available kits with standards ranging from 0.025 to 6.4 ppb.

The MaxSignal Nitrofurantoin (AHD) ELISA Kit is based on a competitive colorimetric ELISA assay. The AHD antibody has been coated in the plate wells. During the analysis, sample is added along with the AHD-horseradish peroxidase (AHD-HRP) conjugate. If the AHD residue is present in the sample, it will compete for the AHD antibody; thereby, preventing the AHD-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the AHD residue concentration in the sample.


One Extraction, Analyze Multiple Nitrofurans

 

To reduce the time and costs associated with sample extraction, Bioo Scientific has developed a single protocol that can be used for testing samples with all of our ELISAs for the detection of nitrofuran antibiotics. Now you can take one sample, perform one extraction and test for AHD, SEM, AMOZ and AOZ.

Nitrofurantoin belongs to the class of 5-nitrofuran antibiotics and has been widely and effectively used for the prevention and treatment of gastrointestinal infections caused by Escherichia coli and Salmonella spp. in cattle, pigs and poultry. It was also used as a growth promoter in food-producing animals. However, both WHO and the European Union (EU) are unable to assign a maximum residue limit for nitrofurantoin because of the potential carcinogenic effects of its residues on human health. As a consequence, the administration of nitrofurantoin to food-producing animals has been prohibited. Because of the rapid excretion of the nitrofurans and their instability in vitro and in vivo, it is impossible to monitor residues of the parent drug nitrofurantoin directly. Alternatively, 1-aminohydantoin (AHD), the major metabolite of nitrofurantoin, which is stable in tissue, even after long-term storage, is selected to monitor.

This kit is manufactured to the international quality standard ISO 9001:2008.


Kit Specs

PLATE: 1 x 96 well (Removable 8 wells x 12 strips) 

MEASUREMENT: Microtiter Plate Reader with 450 nm wavelength (Bioo Cat# 6003-01)

STANDARDS (PPB): 0 / 0.025 / 0.1 / 0.4 / 1.6 / 6.4

TESTS PER KIT: The kit has the capacity for 96 determinations of 42 samples in duplicate (assuming 12 wells for standards).

STORAGE: Store all kit components at 2 – 8 °C. Certain components should be stored at - 20 °C if longer storage is required. See kit manual for more details.

SHELF LIFE: 12 months with proper storage

MaxSignal® Nitrofurantoin (AHD) ELISA Detection Limits

Cat # Product Feed (ppb) Fish/Shrimp/Meat (ppb) Egg (ppb) Egg Powder (ppb) Honey (ppb) Milk (ppb)
1070-02F MaxSignal® Nitrofurantoin (AHD) ELISA Kit 0.1 0.05 0.05 0.05 0.05 0.05