Offers a rapid and cost-effective extraction methods for various samples
- High sensitivity
- Low detection limit
- Quick ELISA assay - less than 1.5 hours regardless of number of samples
- High reproducibility
ELISA for the Screening of Nitrofurazone Residues
The MaxSignal® Nitrofurazone (SEM) ELISA Kit provides a competitive enzyme immunoassay for the quantitative analysis of nitrofurazone in a variety of sample types.
The MaxSignal® Nitrofurazone (SEM) ELISA Kit is based on a competitive colorimetric ELISA assay. SEM antibody has been coated in the plate wells. During the analysis, sample is added along with the SEM-horseradish peroxidase (SEM-HRP) conjugate. If the SEM residue is present in the sample, it will compete for the SEM antibody, thereby preventing the SEM-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the SEM residue concentration in the sample.
Nitrofurazone belongs to the class of 5-nitrofuran antibiotics and has been widely and effectively used for the prevention and treatment of gastrointestinal infections caused by Escherichia coli and Salmonella spp. in cattle, pigs and poultry. It was also used as a growth promoter in food-producing animals. However, both WHO and the European Union (EU) are unable to assign a maximum residue limit for nitrofurazone because of the potential carcinogenic effects of its residues on human health. As a consequence, the administration of nitrofurazone to food-producing animals has been prohibited. Because of the rapid excretion of the nitrofurans and their instability in vitro and in vivo, it is impossible to monitor residues of the parent drug nitrofurazone directly. Instead, semicarbazide (SEM), the major metabolite of nitrofurazone, which is stable in tissue even after long-term storage, is selected to monitor.
This kit is manufactured to the international quality standard ISO 9001:2008.
PLATE: 1 x 96 well (Removable 8 wells x 12 strips)
MEASUREMENT: Microtiter Plate Reader with 450 nm wavelength (Bioo Cat# 6003-01)
STANDARDS (PPB): 0 / 0.025 / 0.1 / 0.4 / 1.6 / 6.4
TESTS PER KIT: The kit has the capacity for 96 determinations of 42 samples in duplicate (assuming 12 wells for standards).
STORAGE: Store all kit components at 2 – 8 °C. Certain components should be stored at - 20 °C if longer storage is required. See kit manual for more details.