The detection limits of this kit meet all of the EU MRLs for fluoroquinolones
- Rapid strip test method – 4 minutes
- Broad-spectrum antibody that requires no heating step
- Works in cold milk
- No sample preparation
- High sensitivity
- High reproducibility
- Interpretation of results can be done by eye or using the QuickSTAR™ Strip Reader
Rapid Lateral Flow Strip Test Kit for the Screening of Milk for Fluoroquinolone Residues
The AuroFlow™ Fluoroquinolone Strip Test Kit for Milk is a qualitative and rapid lateral flow assay designed to detect fluoroquinolone antibiotic residues in raw commingled cow’s milk. This state-of-the-art test uses a broad-spectrum antibody capable of specifically detecting a range of fluoroquinolone residues. This test is designed for rapid field use or reference lab settings and eliminates the need for a heating block.
The assay uses a competitive colloidal gold based format. The milk sample is added to a clear plastic reaction vessel, and used to resuspend the lyophilized reagents to a uniform pink color in the bottom of the microtiter wells. The milk is incubated briefly (3 minutes) to allow the fluoroquinolone antibody on the gold particles to engage with any fluoroquinolone antibiotic residues present in the milk. The test strip is then inserted into the sample well with the arrows pointing downward, initiating capillary flow up the strip. Any gold particles that are not complexed with antibiotics present in the milk will bind to the fluoroquinolone antibiotic imprinted at the Test line (T-line), forming a signal (red line) at that position. If the fluoroquinolone antibody on the gold particle has engaged with antibiotic present in the milk sample, the gold particle will flow past the T-line and reach the Control line (C-line). For visual interpretation of the test results, T-line signal intensity that is stronger than the signal at the C-line indicates a negative result. Signal at the T-line which is equal or less intense compared to the C-line indicates the presence of fluoroquinolone antibiotics in the milk. The greater the reduction in signal intensity at the T-line, the greater the concentration of fluoroquinolone antibiotic residues present in the milk.
Fluoroquinolones are a group of antibiotics that can inhibit the activity of bacterial DNA gyrase. The fluoroquinolone group mainly includes enrofloxacin and its active metabolite ciprofloxacin, danofloxacin, difloxacin, norfloxacin, pipemidic acid, ofloxacin, benofloxacin and others. One of widely used fluoroquinolones is enrofloxacin, which was the first fluoroquinolone antimicrobial to be used in veterinary infections by E. coli, Salmonella, Pasteurella, Mycoplasma and Hemophilus species. The MRLs for the sum of enrofloxacin and its active metabolite ciprofloxacin have been stated at 100 μg/kg (ppb) for muscle tissue and fat, and 100 μg/kg (ppb) for milk.
This kit is manufactured to the international quality standard ISO 9001:2008.
The AuroFlow™ Fluoroquinolone Strip Test Kit for Milk has the capacity for 96 (Cat # 1102-01) or 192 (Cat # 1102-02) determinations. The shelf life is 24 months when the kit is properly stored.
The positive and negative control vials contain lyophilized raw milk samples, and must be reconstituted with 1 mL of distilled water before use. The reconstituted raw milk controls are stable for 48 hours at 4°C. Additional positive and negative control vials are available separately (Cat # 110210).