- Fast, low-cost sample prep protocol (10 minutes - 1 hour)
- High recovery rates
- High sensitivity (0.2 ppb)
- Rapid assay protocol (less than 2 hours to process 40 samples in duplicate)
- High reproducibility (interplate and intraplate CV typically < 8%)
Sensitive Amoxicillin ELISA
The MaxSignal® Amoxicillin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of amoxicillin in milk, urine, meat, liver, kidney, muscle, serum, and plasma samples. The MaxSignal Amoxicillin ELISA Test Kit incorporates a fast, low-cost sample prep protocol with high recovery rates.
Amoxicillin ELISA Overview
Amoxicillin has been coated in the plate wells. The sample to be tested is mixed with a high-affinity amoxicillin capture protein and added to the well. If amoxicillin residue is present in the sample, it will compete for the capture protein, thereby preventing the capture protein from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the capture protein bound to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample. The levels of amoxicillin in the sample are determined by comparison of the color intensity of unknown samples with the standard curve, which is made using the amoxicillin standards provided in the kit.
Amoxicillin is widely used to treat bacterial infections in humans and animals. Monitoring of amoxicillin residues in edible tissues and milk is necessary because of the hypersensitivity of some individuals to amoxicillin, and also the emergence of antibiotic-resistant strains of bacteria. Consequently, maximum residue limits have been specified for food products and milk to control the levels of amoxicillin reaching the consumer.
The MaxSignal Amoxicillin ELISA Test Kit has the capacity for 96 determinations, or testing of 42 samples in duplicate (assuming 12 wells for standards). Store the kit at 2-8ºC. The shelf life is 12 months when the kit is properly stored.