- Offers high yields of total RNA from tissues and cells
- Includes protocol for enrichment of microRNA
- Works well for recovering RNA from cell-free fluids
- Includes inert co-precipitant for maximizing RNA recovery
The BiooPure™ RNA Isolation Reagent is a single-phase reagent for extraction of total RNA or enriched small RNA (including miRNA) from solid tissues, cultured cells, and cell-free fluids such as serum and plasma. The BiooPure Reagent contains guanidinium, a powerful chaotropic agent effective for rapidly inactivating nucleases, and phenol, an organic solvent used to denature and separate proteins and DNA from RNA.
Selected Publications that Cite Use of BiooPure:
Burgos K, Javaherian A, Bomprezzi R. et al.(2013) Identification of extracellular miRNA in human cerebrospinal fluid by next-generation sequencing. RNA Journal. (5):712-22. doi: 10.1261/rna.036863.112.
Huanga, R.-Y., Sub, S.-G., Wuc, D.-C. and Fud, J. (2015) BLZF1 expression is of prognostic significance in hepatocellular carcinoma. Biochem. Biophy. Res. Comm. doi:10.1016/j.bbrc.2015.08.119.
Luo R, Zhang M, Liu L, Lu S, Zhang CZ, et al. (2013) Decrease of Fibulin-3 in Hepatocellular Carcinoma Indicates Poor Prognosis. PLoS ONE 8(8): e70511. doi:10.1371/journal.pone.0070511.
Liu L, Zhang CZ, Cai M, Fu J, Chen GG, et al. (2012) Downregulation of Polo-Like Kinase 4 in Hepatocellular Carcinoma Associates with Poor Prognosis. PLoS ONE 7(7): e41293. doi:10.1371/journal.pone.0041293.
Nakashe, P. et al. (2011) Adapter Dimer Reduction in High-Throughput microRNA Profiling. OMICS 1(1): 6-11. http://www.asciencejournal.net/asj/index.php/OMICS/article/view/290/245
Shende V.R., Goldrick M.M., Ramani S. and Earnest D.J. (July 22, 2010) Expression and Rhythmic Modulation of Circulating MicroRNAs Targeting the Clock Gene Bmal1 in Mice. PLoS ONE 6(7): e22586. doi:10.1371/journal.pone.0022586
Xu SJ, Zhang R, et al. (2012) Oxidative Stress Mediated-Alterations of the MicroRNA Expression Profile in Mouse Hippocampal Neurons. International Journal of Molecular Sciences. doi:10.3390/ijms131216945.
You, Z., et al. (2015) Activating transcription factor 2 expression mediates cell proliferation and is associated with poor prognosis in human non-small cell lung carcinoma. Oncology Letters. doi: 10.3892/ol.2015.3922.
Zhang, J. et al. (2012) FRS2α-Mediated FGF Signals Suppress Premature Differentiation of Cardiac Stem Cells Through Regulating Autophagy Activity. Circulation Research. doi: 10.1161/CIRCRESAHA.111.255950.
Zhang CZ, Liu L, Cai M, Pan Y, Fu J, et al. (2012) Low SIRT3 Expression Correlates with Poor Differentiation and Unfavorable Prognosis in Primary Hepatocellular Carcinoma. PLoS ONE 7(12): e51703. doi:10.1371/journal.pone.0051703.
Zheng, Z., Liu, X., Zhao, Q., Zhang, L., Li, C. and Xue, Y. (2014) Regulation of UCP1 in the Browning of Epididymal Adipose Tissue by β3-Adrenergic Agonist: A Role for MicroRNAs. International Journal of Endocrinology. 530636, doi: 10.1155/2014/530636.
Zhou, H., et al. (2016) Deregulation of miRNA-181c potentially contributes to the pathogenesis of AD by targeting collapsin response mediator protein 2 in mice. Journal of the Neurological Sciences. doi: 10.1016/j.jns.2016.05.038.
The BiooPure RNA Isolation Reagent contains enough reagents for processing up to 10 grams of solid tissue or approximately 108 – 109 eukaryotic cells.