Detect Total Homocysteine Levels in Animal Serum
The MaxDiscovery™ Homocysteine Enzymatic Assay Kit, optimized for preclinical testing, uses a novel enzymatic technology with high sensitivity and low detection limits for spectrophotometric detection of total homocysteine levels in serum. This end point assay facilitates in vitro quantitative determination of total L-Homocysteine (tHcy) using only 20 µL of serum samples, making it ideal for use on rodents and other model organisms. This flexible assay can be performed manually for low throughput analysis and automated for high throughput requirements.
Total Homocysteine Detection Procedure Overview
The MaxDiscovery Homocysteine Enzymatic Assay is based on a novel enzymatic principle that measures the co-substrate conversion product. In the assay, the oxidized homocysteine is first reduced to free Hcy. Reduced Hcy undergoes an enzymatic reaction to produce S-adenosyl homocysteine (SAH), a co-substrate conversion product. This reaction is catalyzed by homocysteine S-methyltransferase. S-adenosyl homocysteine in the presence of enzyme S-adenosyl homocysteine hydrolase produces free Hcy and adenosine. Adenosine undergoes deamination to form inosine and ammonia. Ammonia acts as a substrate for the GLDH enzyme, as a result of which NADH is oxidized to NAD+. The levels of free Hcy are directly proportional to the amount of NAD+ (absorbance at 340nm) detected in the sample.
MaxDiscovery Homocysteine Enzymatic Assay Kit Specs
The MaxDiscovery Homocysteine Enzymatic Assay Kit has the capacity for 48 determinations or testing of 24 samples in duplicate (assuming 12 wells for standards). Store the kit at 2 – 8°C. The shelf life is 12 months when the kit is properly stored.