High sensitivity with low detection limit
- Easily integrated into existing cell culture parameters
- User-friendly manual
- High compatibility across a broad range of cell lines
- Can be used with media containing high serum concentrations with confidence
- A robust enzyme-based assay
- Does not require expensive instrumentation
- Highly reproducible results
- Specially formulated BIOO Booster reagent significantly improves assay signal
- Measure absorbance in UV spectrum (340 nm)
The MaxDiscovery™ Aspartate Transaminase (AST) Cytotoxicity Kit is a plate-based colorimetric enzymatic assay for the determination of the aspartate transaminase enzyme in cultured cell lines. Aspartate transaminase (AST) also known as aspartate aminotransferase or (sGOT) is a metabolic enzyme expressed in a host of mammalian cell types. Upon cell damage or lysis the enzyme is released into the cell culture supernatant and is thus a useful tool for the investigation of cytotoxicity effects of experimental drug formulations and cell transfection.
The MaxDiscovery™ Alanine Transaminase (AST) Cytotoxicity Assay Kit uses a coupled enzymatic reaction scheme: aspartate and α-ketoglutarate are first converted to glutamate and oxaloacetate which is converted by malate dehydrogenase to make malate and NAD+. The conversion of the NADH chromophore to NAD+ product, measured at 340 nm, is proportional to the level of AST enzyme in the sample. The absorbance of each well at 340 nm is measured using a plate reader. The concentration of AST in each sample is then directly determined from the change in absorbance at 340 nm over a 30 minute assay length.
Although AST is present in a host of human tissues, its release varies among cell types. Therefore, the most useful analysis of cytotoxicity in a cultured cell line involves measuring cytotoxicity as a percentage of damaged or lysed cells.
The MaxDiscovery™ Aspartate Transaminase (AST) Cytotoxicity Assay Kit has the capacity for 192 determinations or testing of 84 samples in duplicate (using 12 wells for standards). The kit also contains enough material to construct four standard curves. Store the kit at 4°C. The shelf life of the kit is 4 months, after receipt, when the kit is properly stored.