- High sensitivity
- High compatibility across a broad range of cell lines
- A robust enzyme-based assay which does not require expensive instrumentation
- High reproducibility
The MaxDiscovery™ Caspase-3 Colormetric Detection Kit is a colorimetric microplate-based assay to directly measure caspase-3 activation in cultured cells. This kit enables biomedical researchers to determine caspase-3 levels in cultured cells from a broad range of cell lines. The assay utilizes a simple, proven enzymatic assay to detect activated enzyme.
Caspase-3 is a ubiquitously-expressed cysteine protease involved in programmed cell death (apoptosis). In nonapoptotic cells, this enzyme exists as an inactive zymogen known as procaspase-3. Intracellular procaspase-3 is activated when cells are committed to die via apoptosis; induction of the cell death machinery eventually triggers activation of the zymogen by proteolytic cleavage of the zymogen to an active heterodimer comprised of 17 kDa and 12 kDa subunits. The active enzyme cleaves a number of intracellular protein targets important for cell survival. Therefore, monitoring caspase-3 enzyme activity is a fundamental means to measure apoptosis in cells. This assay is an important tool for apoptosis research as well as investigating the apoptotic effects of experimental drug formulations and cell transfection.
Because caspase-3 is expressed in every cell type, the assay can be performed using any mammalian cell line.
The MaxDiscovery™ Caspase-3 Detection Kit has the capacity for 96 determinations or testing of 48 samples in duplicate. Upon receipt of the kit, store it at -20°C; the microtiter plate can be stored at -20°C, 4°C or room temperature. When properly stored, the kit will maintain activity for 12 months after receipt.