MaxSignal® Fructose Assay Kit

Catalog# Product Name Quantity US List Price
1309-01 MaxSignal® Fructose Assay Kit
48 samples
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These kits are available for purchase by email, fax or phone only. Please email your order to bioo.sales@perkinelmer.com, fax it to 1-512-707-8122 or call 512-707-8993.


  • High sensitivity and low detection limit
  • A rapid, robust enzyme-based assay complete in 30 minutes
  • High reproducibility
  • Flexible assay format

The MaxSignal® Fructose Assay Kit is a cuvette-based enzymatic assay for the determination of fructose across a variety of sample types. The assay is useful for analysis of inverted sugar, a mixture of equal parts of glucose and fructose resulting from the hydrolysis of sucrose; this sugar is found naturally in fruits and honey and produced artificially for use in the food industry. The assay can also be performed using a microtiter plate for high-throughput sample analysis.

The MaxSignal® Fructose Assay Kit uses a highly-specific coupled enzymatic reaction to detect fructose in food samples: Fructose is first specifically converted to fructose-6-phosphate (F-6-P) using hexokinase. Next the F-6-P is converted to glucose-6-phosphate (G-6-P) using the enzyme phosphoglucose isomerase (PGI). The G-6-P compound is then used as a substrate by the enzyme glucose-6-phosphate dehydrogenase to convert NADP+ to NADPH. This conversion, measured at 340 nm, is proportional to the level of fructose in the sample.

The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of fructose in each sample is then directly determined from the change in absorbance at 340 nm within 30 minutes.

This kit is manufactured to the international quality standard ISO 9001:2008.


Kit Specs

The MaxSignal Fructose Assay Kit has the capacity for 48 determinations, or 24 determinations in duplicate. Store the kit at 4°C.