NextFect™ DNA Transfection Reagent

The ultimate in high efficiency and low toxicity

Catalog# Product Name Quantity US List Price
340301 NextFect™ DNA Transfection Reagent 0.9 mL $109 Buy Now
340302 NextFect™ DNA Transfection Reagent 1.8 mL $219 Buy Now
340303 NextFect™ DNA Transfection Reagent 9 mL $874 Buy Now
340304 NextFect™ DNA Transfection Reagent 27 mL $2,295 Buy Now



  • Compatibility with a broad range of cell types; adherent and suspension cells
  • Simple, rapid protocol with fewer steps for consistent & reproducible results
  • Easy to optimize due to excellent cell viability
  • Reverse or forward transfection method
  • Superior adaptability to high throughput (HT) systems 

NextFect™ DNA Transfection Reagent is a new generation of transfection reagent optimized for nucleic acid delivery into eukaryotic cells. Its proprietary formulation of novel lipid and peptide compositions makes it a superior non-liposomal reagent for DNA transfection into a broad range of cell types. Due to its low cytotoxicity, NextFect DNA Transfection Reagent is highly suitable for transfection of primary cells and many sensitive cell lines. 

Higher transfection efficiencies of plasmid DNA are achieved with NextFect DNA Transfection Reagent than with other reagents when used with the recommended procedures. Many cell lines and primary cells have been successfully transfected using NextFect DNA Transfection Reagent; cell type-specific transfection protocols are available. 

NextFect DNA Transfection Reagent was used for expression of recombinant viral proteins in 293f suspension cells. Stable cell lines expressing the non-toxic viral protein were successfully generated. 

The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. Transfection using NextFect DNA Transfection Reagent requires minimal handling and is highly suitable for high-throughput screening. NextFect DNA Transfection Reagent is available in bulk quantities. 

Cytotoxicity with NextFect DNA Transfection Reagent is minimal because the lipid and peptide components of the formulation have been optimized after rigorous screening over several cell lines. Thus this transfection reagent is a superior alternative to traditional techniques including calcium phosphate co-precipitation, electroporation, microinjection, biolistic particle delivery, and complex formation with DEAE-dextran. 

NextFect DNA Transfection Reagent provides excellent performance in both stable and transient transfection of mammalian cells and is ideal for use with reporter (pMLuc™), drug selection (pcDNA4™ and pTandem™-1), vectors. 

Additionally, the NextFect DNA Transfection Reagent has been shown to support robust shRNA induced silencing. Transfection is performed in chemically defined media and does not contain any animal-derived components. 

The 0.9-mL size provides enough reagent to perform up to 90 transfections in a 48-well plate. NextFect DNA Transfection Reagent is supplied as a ready-to-use sterile solution. 

Kit Specs

QUALITY CONTROL Tested for DNase and RNase activity and cell culture functional performance. 
STORAGE Store at 4°C upon arrival. Do not freeze. 
SHELF LIFE Shelf life is 6 months when stored correctly