Dr. Masoud Toloue will Describe Methyl-Seq, PCR-Free and Small RNA-Seq NGS Library Prep Technologies

• Updated:06-28-2011
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• Dr. Masoud Toloue, Director of Genomic Research at Bioo Scientific, will describe the latest DNA and RNA library preparatory methods for next-generation sequencing at the upcoming European Next Generation Sequencing Congress in Hamburg, Germany. Strategies for massive parallel sequencing have revolutionized research across diverse scientific disciplines. Despite these advances, DNA and RNA sample preparations, one of the most important aspects of next generation sequencing, continue to use outdated, biased and cumbersome methods. However, recent improvements in library preparation technology have improved genome sequencing.
  
  Bioo Scientific is commitment to developing innovative solutions to improve, simplify and reduce bottlenecks in next generation sequencing. Fragmentation, ligation and amplification methods typically used in next generation sequencing library preparation are susceptible to sequence bias, require significant preparatory time and are highly inefficient. In order for genomic sequencing to become a staple in the clinic, steps need to be taken to improve this. To circumvent these obstacles Bioo Scientific has developed several strategies for RNA–Seq that not only eliminate small RNA circularization and linker dimers, but also allow for cross genome analysis using multiplexed barcodes. Improvements to DNA preparations include a series of coordinated reactions designed to maximize ligation yield while not compromising on efficiency. This work has directly resulted in the development of a method to successfully prepare libraries without any PCR amplification, a crucial advance when seeking non biased data.
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