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MaxDiscovery™ Alanine Transaminase (ALT) Enzymatic Assay Kit
 
 
Catalog# Product Name Quantity US List Price
3460-01
MaxDiscovery™ Alanine Transaminase Enzymatic Assay Kit
1 x 96 wells $276
 
 
   Benefits  
· High sensitivity with low detection limit (20 U/L)
· Rapid – results in 5 minutes
· Robust kinetic assay
· Does not require expensive instrumentation
· Highly reproducible results
· Only requires 10 uL of serum
· Measure absorbance in UV spectrum (340 nm)
 
 
   Description  
The MaxDiscovery™ Alanine Transaminase (ALT) Enzymatic Assay Kit is a plate-based colorimetric enzymatic assay for the determination of the alanine transaminase enzyme in 10 µL of serum from rodents or other mammals. Alanine transaminase (ALT) (also known as alanine aminotransferase or sGPT) is a metabolic enzyme expressed primarily in the liver. Damage to the liver causes the release of this enzyme into the blood. Elevation of ALT levels is an indication of liver damage and has been associated with liver injury. ALT levels are monitored routinely in patients with liver diseases. ALT is also a very useful tool for preclinical investigation of experimental drug formulations and ALT levels are commonly used to monitor and attenuate the hepatotoxic effects of experimental drugs in rodents.
 
 
   Key Features  
The MaxDiscovery™ Alanine Transaminase (ALT) Enzymatic Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for standards). The kit also contains enough material to construct four standard curves. Store the kit at 4°C. The shelf life of the kit is 12 months when properly stored. Once the Reagent Mix is reconstituted the shelf life of the kit is 3 months when properly stored.

Selected References:

Fang X, Wang R, Ma J, Ding Y, Shang W, et al. (2012) Ameliorated ConA-Induced Hepatitis in the Absence of PKC-theta. PLoS ONE 7(2): e31174. doi:10.1371/journal.pone.0031174

Kühnel, F. et al. (29 December 2009) Targeting of p53-Transcriptional Dysfunction by Conditionally Replicating Adenovirus Is Not Limited by p53-Homologues. Mol. Ther.

Wu, S.Y. et al. (12 August 2010) Systemic delivery of E6/7 siRNA using novel lipidic particles and its application with cisplatin in cervical cancer mouse models. Gene Therapy.

Kverka, M. et al. (May 2011) Safety and efficacy of the immunosuppressive agent 6-tioguanine in murine model of acute and chronic colitis, BMC Gastroenterology, 11 (1) 47.

Hu, S. et al. (July 6, 2011) 13C-Pyruvate Imaging Reveals Alterations in Glycolysis that Precede c-Myc-Induced Tumor Formation and Regression. Cell Metabolism 14 (1): 131 – 142.

Patel R, Baker SS, Liu W, Desai S, Alkhouri R, et al. (2012) Effect of Dietary Advanced Glycation End Products on Mouse Liver. PLoS ONE 7(4): e35143. doi:10.1371/journal.pone.0035143

Siegemund, M. et al. (April 2012) Superior antitumoral activity of dimerized targeted single-chain TRAIL fusion proteins under retention of tumor selectivity. Cell Death and Disease (2012) 3, e295; doi:10.1038/cddis.2012.29

 
 
 
 
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   Notes  
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
 

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