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The MaxRecovery™ Nucleic Acid Extraction Kit is designed for extraction of total nucleic acid (DNA and RNA) from small amounts (~10 – 20 mg) of solid tissue. The first step is to grind or otherwise disrupt the tissue in Lysis Solution containing SDS and Proteinase K. The lysate is then incubated at high temperature to digest proteins and release nucleic acid, followed by removal of proteins and cellular debris by precipitation and centrifugation. The nucleic acid is then recovered from the clarified lysate by isopropanol precipitation. The kit has been used to extract cellular and viral DNA and RNA. Yields are dependent on the type and amount of tissue, but are typically between 10 – 100 ug per prep. The nucleic acid recovered using this kit is especially useful for PCR. The RNA may not be of sufficient quality for applications that require highly intact RNA. The purity of the nucleic acid as determined by UV absorbance ratio (Abs 260 / Abs 280) is typically between1.8 – 2.0. |
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