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CONCENTRATION: 200 U/uL
UNIT DEFINITION: One unit is defined as the amount of the enzyme required to give 50% ligation of a 17-mer adenylated oligonucleotide to a purified control RNA template in 30 minutes at 37°C.
SOURCE: Purified from an E. coli strain carrying T4 RNA Ligase 2 truncated overproducing plasmid.
STORAGE: Store at -20°C
SHELF LIFE: 1 year when stored under optimal conditions
STORAGE & DILUTION BUFFER: 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 0.1 mM DTT and 50% Glycerol
QUALITY CONTROL: Enzyme preparations are routinely assessed for relative purity, activity and absence of RNase and DNase. In addition, this enzyme is tested for reactivity with adenylated linkers (catalog # 510205, 510305, 510405)
Validated on the Illumina Solexa GAII sequencing platform
REFERENCES: Ho, C.K. et al. (2004) Structure, 12, 327-339.
Ho, C.K. and Shuman, S. (2002) Proc. Natl.Acad.Sci. USA, 99, 12709-12714. |
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