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For Small RNA Next-Generation Sequencing |
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Catalog# |
Product Name |
Quantity |
US List Price |
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510501
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AIR™ Barcoded Adapters Set 1: 10 Adapters
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25 rxns each |
$2,990 |
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510502
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AIR™ Barcoded Adapters Set 2: 20 Adapters
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25 rxns each |
$5,350 |
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510503
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AIR™ Barcoded Adapters Set 3: 30 Adapters
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25 rxns each |
$7,600 |
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• Considerably reduce your small RNA per-sample sequencing cost by barcoded multiplexing
• Design experiments with multiple samples, conditions and time-courses
• Increase your sequencing scale
• Pool multiply libraries into a single reaction
• Identify undiscovered miRNAs
• Maximize your flow cell
• Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq)
• SOLiD barcodes are available as custom orders
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AIR Barcoded Adapters enable the user to detect rare small RNA sample events amongst hundreds of samples, across several time points and even multiple genomes. Barcode sets were chosen based on uniform melting temperature (Tm) and sequences with unique color space. AIR Barcoded Adapters are pre-adenylated 3’ adapters that are blocked at their 3’ends. This eliminates the need for ATP during ligation, minimizing ligation to the 5’ phosphate of the small RNA pool and subsequent circularization.
The barcoded adapters allow multiplexing of small RNA sequencing samples and can be used with the AIR Small RNA Sequencing Kit or with your own small RNA sample prep procedure. AIR Small RNA Sequencing Kit offers numerous advantages over the Illumina kit. It uses a faster procedure, it incorporates fewer gel purifications, it is compatible with multiplexing, it offers greater sequencing coverage and it is less expensive. I attached a copy of the manual to this email.
Additionally, we can develop Custom AIR Adapters if you want to design your own barcodes. SOLiD barcodes are available as custom orders.
Multiplex tagging has the potential to spread the capacity of Next Generation Sequencing across several genomes.
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