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The MaxDiscovery™ Triglycerides Enzymatic Assay Kit is a plate-based colorimetric enzymatic assay for the determination of triglycerides in serum samples. Triglycerides are a prominent class of lipids found in blood. Triglycerides are used to form cell membranes and hormones, and play important roles in signal transduction pathways. The determination of serum triglycerides is an important marker for the diagnosis of lipemias. Elevated levels of triglycerides are strongly associated with vascular diseases such as stroke and atherosclerosis.
MaxDiscovery™ Triglycerides Enzymatic Assay Kit is a simple, direct and automation-
compatible method for measuring triglyceride levels in serum samples. This kit uses a coupled enzymatic reaction scheme: triglyceride esters are first converted to glycerol and fatty acids. Next, glycerol is converted using ATP to glycerol-phosphate. Lastly, the glycerol-phosphate is enzymatically oxidized to form hydrogen peroxide, which is visibly detected (at 520 nm) by peroxidase using a chromogenic dye. The absorption measured at 520 nm, is proportional to the concentration of triglycerides in the sample. The kit also comes with a control solution containing a reference standard (200 mg/dL), which can be used to calibrate the assay.
This kit provides direct determination of triglycerides in serum, plasma, and other fluid samples. In addition, the kit can be used to analyze the pharmacological effects of drugs on triglycerides metabolism.
Selected Reference:
Evans, J.F. et al. (2012) The lipocalin-type prostaglandin D2 synthase knock-out (L-PGDS KO) mouse model of insulin resistance and obesity demonstrates early hypothalamic-pituitary-adrenal (HPA) axis hyperactivity. Journal of Endocrinology. doi: 10.1530/JOE-12-0275 |
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