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The MaxDiscovery™ LDL Cholesterol Assay Kit is a simple microplate-based method for measuring LDL cholesterol levels from rodent serum or plasma. This kit uses a specific detergent formulation to selectively dissolve non-LDL lipoprotein particles (HDL, VLDL and chylomicrons) while leaving LDL particles intact. The dissolved cholesterol is degraded by the cholesterol esterase and cholesterol oxidase enzymes. Next a second detergent is added to the sample to solubilize the remaining LDL particles. The soluble cholesterol and cholesterol esters are oxidized by cholesterol oxidase to produce hydrogen peroxide. The hydrogen peroxide product then reacts with bis(4-sulfobutyl)-m-toluidine and 4-aminoantipyrine to form a colored product. The resulting color change is measured at 550 nm and is proportional to the amount of LDL cholesterol originally present in the sample.
The kit also comes with a control solution containing a LDL cholesterol standard which can be used to calibrate the assay and generate a standard curve.
LDL (low-density lipoprotein) is one type of lipid/protein particle used to transport lipids such as triglycerides and phospholipids throughout the bloodstream. LDL particles also contain significant amounts of cholesterol. Increased levels of LDL cholesterol are associated with atherosclerosis and coronary artery disease. For this reason, measuring LDL cholesterol levels has been shown to often predict the occurrence of heart disease.
Selected Reference:
Evans, J.F. et al. (2012) The lipocalin-type prostaglandin D2 synthase knock-out (L-PGDS KO) mouse model of insulin resistance and obesity demonstrates early hypothalamic-pituitary-adrenal (HPA) axis hyperactivity. Journal of Endocrinology. doi: 10.1530/JOE-12-0275 |
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