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AIR™ Paired-End DNA Sequencing Kit
 
 
Catalog# Product Name Quantity US List Price
5137-01
AIR™ Paired-End DNA Sequencing Kit
10 rxns $392
5137-02
AIR™ Paired-End DNA Sequencing Kit
40 rxns $1,273
 
 
   Benefits  
• Paired-end sequencer-ready libraries from as low as 1 µg of DNA
• Includes all enzymes, adapters and clean-up columns required
• Read both forward and reverse template strands
• Single step blunt-end polishing
• Single day library construction
• Optimized for small and large DNA inserts
• Cost effective and flexible sequencing preparation
• Indexing/barcoding compatible
 
 
   Description  
The AIR™ Paired-End DNA Sequencing Kit is ideally suited for preparation of expression libraries that allow reads to be generated from both ends of the library template. The benefits of this include more sequence information, positional information when mapping to a reference genome and accurate alignments. The kit is designed for next-generation sequencing on Illumina platforms.

The AIR™ DNA Sequencing Kits offer significant improvements over the traditional library preparation methods, including their ability to produce high quality, unbiased, sequencer-ready libraries from as little as 1 µg of DNA. They are complete kits that include all enzymes, adapters and clean-up columns needed for library preparation, facilitating the user’s ability to move from purified genomic DNA to cluster generation. Each kit has been functionally validated with the Illumina GAII, HiSeq and MiSeq sequencing platforms. These kits are designed for use in de novo sequencing and targeted re-sequencing applications.

DNA Fragmentation
Both the AIR™ Paired-End DNA and the AIR™ Genomic DNA Sequencing Kits are compatible with the AIR™ DNA Fragmentation Kit that provides a means for unbiased DNA fragmentation. These kits are also compatible with alternative DNA shearing technologies such as ultrasound and sonication.

Sample Multiplexing
AIR™ DNA Barcodes can be used to provide flexibility in high-throughput sequencing applications. They significantly increase scale and throughput while reducing costs by allowing the user to pool up to 96 multiple paired end library preparations into a single flow cell.

For larger volume requirements, customized and bulk packaging is available. Please contact nextgen@biooscientific.com for further information.
 
 
   Key Features  
The AIR Paired-End DNA Sequencing Kit contains enough material to prepare 10 or 40 genomic DNA samples for Illumina® compatible next-generation sequencing. The shelf life of all reagents is 12 months when stored at -20°C.

Kit Components:
AIR™ 10X End Repair Buffer
AIR™ End Repair Enzyme Mix
dNTPs
AIR™ 10X 3’ Adenylation Buffer
dATP
Klenow Fragment (3’-5’exo-minus)
PE Adapter Oligos*
AIR™ 2X Rapid Ligase Buffer
Rapid T4 DNA Ligase
PE Primer 1
PE Primer 2
5X DuroTaq Master Mix
6X Loading Dye
MW Ladder Ready-to-Load- 100 bp
Nuclease-free Water
Clean-Up Spin Columns
Column Elution Buffer
Binding Buffer
Wash Buffer

 
 
 
 
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  Related Product/Services
AIR™ DNA Barcodes
AIR™ DNA Fragmentation Kit
AIR™ Genomic DNA Sequencing Kit
 

 
   Notes  
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
 

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