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Indexing of DNA samples for NGS on Illumina platforms |
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Catalog# |
Product Name |
Quantity |
US List Price |
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514101
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NEXTflex™ DNA Barcodes - 6
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48 rxns |
$414 |
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514102
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NEXTflex™ DNA Barcodes - 12
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96 rxns |
$817 |
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514103
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NEXTflex™ DNA Barcodes - 24
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192 rxns |
$1,618 |
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514104
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NEXTflex™ DNA Barcodes - 48
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384 rxns |
$3,183 |
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• Index contained within adapter sequence
• Up to 48 multiplexed samples
• Considerably reduce your per-sample sequencing cost by barcoded multiplexing
• Increase your sequencing scale by pooling 100s of samples on a single flow cell
• Compatible with Illumina Next-Generation Sequencing platforms (GAIIx, HiSeq and MiSeq) |
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NEXTflex™ DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences.
These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits and other genomic DNA library prep protocols.
Do you want to index 96 samples? We also offer the NEXTflex-96™ DNA Barcode kits, which contain 96 - 8 nt index barcodes in either a 96-well format (cat # 514105) or in microfuge tubes (cat # 514106).
The NEXTflex™ RNA-Seq Barcodes and NEXTflex-96™ RNA-Seq Barcodes are now available for your RNA-seq multiplexing needs.
Download a copy of our Application Note, Tools for Enhanced Sequence Diversity and Reducing Bias in DNA-seq Library Preparation, which was recently published on Nature Methods.
For larger volume requirements, customized and bulk packaging is available. Please contact nextgen@biooscientific.com for further information.
Selected References:
Herron MD, Doebeli M (2013) Parallel Evolutionary Dynamics of Adaptive Diversification in Escherichia coli. PLoS Biol 11(2): e1001490. doi:10.1371/journal.pbio.1001490
Tamburrino, A. et al. (2012) Activation of the mTOR Pathway in Primary Medullary Thyroid Carcinoma and Lymph Node Metastases. Clinical Cancer Research. doi: 10.1158/1078-0432.CCR-11-2700 |
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