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Custom AIR™ Adenylated Linkers |
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For Next-Generation Sequencing |
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• Reduces cost of Next-Generation Sequencing
• Ideal for next-generation sequencing, miRNA cloning and miRNA sequencing
• 3’ end blocks prevent self-ligation
• Adenylated 5' end reacts with T4 RNL2 Ligase, truncated
• Phosphorothioate and other modifications available
• Predesigned linkers are also available
• You can create your own miRNA barcodes
To request a quote for custom adenylated adapters call 512-707-8993 or email us at nextgen@biooscientific.com. |
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Design your own miRNA cloning linkers.
Bioo Scientific’s AIR™ Adenylated Linkers are 5’adenylated oligos with 3’end blocks, which are ready for use in Next-Generation Sequencing or miRNA cloning. Custom adaptors can be designed for use with SOLiD, 454 FLX, Illumina or any other Next-Generation Sequencing platform.
AIR™ Adenylated Linkers are activated adenylated oligos that preclude the need for ATP in RNA Ligase 2 (RNL2) reactions. This allows for specific and clean ligations.
Selected References:
Haenni, S. et al. (March 2012) Analysis of C. elegans intestinal gene expression and polyadenylation by fluorescence-activated nuclei sorting and 3'-end-seq. Nuc Acid Res, 40:6 http://nar.oxfordjournals.org/content/early/2012/03/28/nar.gks282.full |
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