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NEXTflex™ Small RNA Barcode Primers |
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Indexing of Small RNA samples for NGS on Illumina platforms |
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Catalog# |
Product Name |
Quantity |
US List Price |
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513301
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NEXTflex™ Small RNA Barcode Primers (Set A)
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96 rxns |
$424 |
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513302
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NEXTflex™ Small RNA Barcode Primers (Set B)
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96 rxns |
$424 |
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513303
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NEXTflex™ Small RNA Barcode Primers (Set C)
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96 rxns |
$424 |
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513304
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NEXTflex™ Small RNA Barcode Primers (Set D)
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96 rxns |
$424 |
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• Barcoded PCR Primers for multiplexing contain embedded index sequence
• Up to 48 multiplexed samples
• Considerably reduce your per-sample sequencing cost by barcoded multiplexing
• Increase your sequencing scale by pooling 100s of samples on a single flow cell
• Compatible with Illumina Next-Generation Sequencing platforms (GAII, HiSeq and MiSeq) |
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Four sets of 12 NEXTflex™ Small RNA Barcode Primers, with embedded index sequences are available offering an improved multiplexing workflow and increased flexibility. This new automation-friendly format enables multiplexing of up to 48 samples. The ability to pool samples in an efficient way significantly decreases hands on time while providing robust data quality. The primer barcoding system utilizes a 6 nt index to differentiate up to 48 different samples on a single flow cell lane. 48 barcoded primers are divided into 4 different kits (A-D).
These barcodes were designed to be used with the patent pending NEXTflex™ Small RNA Sequencing Kit.
Selected Citations:
Maczuga, P. et al. (Jan 2013) Embedding siRNA sequences targeting Apolipoprotein B100 in shRNA and miRNA scaffolds results in differential processing and in vivo efficacy. Molecular Therapy (2013); 21 1, 217-227. doi: 10.1038/mt.2012.160
Nakashe, P. et al. (2011) Adapter Dimer Reduction in High-Throughput microRNA Profiling. OMICS 1(1): 6-11. |
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