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NEXTflex™ ChIP-Seq Kit
 
 
Catalog# Product Name Quantity US List Price
5143-02
NEXTflex™ ChIP-Seq Kit
48 rxns $1,527
5143-01
NEXTflex™ ChIP-Seq Kit
8 rxns $318
514120
NEXTflex™ ChIP-seq Barcodes - 6
48 rxns $207
514121
NEXTflex™ ChIP-seq Barcodes - 12
96 rxns $409
514122
NEXTflex™ ChIP-seq Barcodes - 24
192 rxns $809
514123
NEXTflex™ ChIP-seq Barcodes - 48
384 rxns $1,591
514124
NEXTflex-96™ ChIP-seq Barcodes - 96
768 rxns $3,077
 
 
   Benefits  
• Ultra low DNA input requirement of 1 ng
• For use with ChIP or genomic DNA samples
• Optimized for low DNA input with NanoQ™ enzymes and buffers
• Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads
• 96 barcoded adapters for multiplexing contain embedded index sequence
• Fast bead-based cleanup protocol shaves hours off of library prep time
• Automation-friendly workflow is compatible with liquid handlers
• Flexible barcode options – kits containing 6, 12, 24, 48 and 96 unique barcodes
• Compatible with Illumina GAIIx, HiSeq and MiSeq sequencing platforms
 
 
   Description  
The NEXTflex™ ChIP-Seq Kit contains specially designed NanoQ™ enzymes and buffers which allow for the ultra low DNA input requirements of only 1 ng for sample preparation of chromatin immunoprecipitated or regular genomic DNA for next generation sequencing. The NEXTflex ChIP-Seq Kit also features Bioo Scientific’s proprietary “Enhanced Adapter Ligation Technology” resulting in library preps with a larger number of unique sequencing reads. By making improvements to the ligation enzymatic mix, the user will now have the ability to perform ligations with longer adapters and expect to see better binding efficiencies.

Simple Automation Friendly Workflow
The NEXTflex ChIP-Seq Kit simplifies the library prep workflow by using master mixed reagents and a gel-free protocol using magnetic bead based cleanup.

Unparalleled Multiplexing Capabilities
Using the NEXTflex™ ChIP-Seq Barcodes, the user can index 6, 12, 24, 48 or even 96 samples at once providing unparalleled ChIP sequencing capacity.

For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available. Please contact nextgen@biooscientific.com for further information.

Select References:

Cvejic A, Haer-Wigman L, Stephens JC et al. (2013) SMIM1 underlies the Vel blood group and influences red blood cell traits. Nature Genetics. doi:10.1038/ng.2603

Thakur J. and Sanyal K. (Feb 2013) Efficient neocentromere formation is suppressed by gene conversion to maintain centromere function at native physical chromosomal loci in Candida albicans. Genome Research. doi: 10.1101/gr.141614.112

Watson E., MacNeil L.T. et al. (2013) Integration of Metabolic and Gene Regulatory Networks Modulates the C. elegans Dietary Response. Cell. http://dx.doi.org/10.1016/j.cell.2013.02.050
 
 
   Key Features  
The NEXTflex ChIP-Seq Kit contains enough material to prepare 8 or 48 ChIP or genomic DNA samples for Illumina® compatible sequencing.

The shelf life of all reagents is 12 months when stored properly. The kit should be stored at -20°C. It ships on dry ice.
 
 
 
 
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  Related Product/Services
NEXTflex™ ChIP-Seq Barcodes
NEXTflex-96™ ChIP-Seq Barcodes
NEXTflex™ DNA Sequencing Kits
 

 
   Notes  
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
 

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