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NEXTflex™ RNA-Seq Kit
for Illumina Sequencing
 
 
Catalog# Product Name Quantity US List Price
5129-01
NEXTflex™ RNA-Seq Kit
8 rxns $299
5129-02
NEXTflex™ RNA-Seq Kit
48 rxns $1,597
512911
NEXTflex™ RNA-Seq Barcodes – 6
48 rxns $207
512912
NEXTflex™ RNA-Seq Barcodes – 12
96 rxns $409
512913
NEXTflex™ RNA-Seq Barcodes – 24
192 rxns $809
512914
NEXTflex™ RNA-Seq Barcodes – 48
384 rxns $1,591
512915
NEXTflex-96™ RNA-Seq Barcodes – 96 (in 96-well plates)
768 rxns $3,077
512916
NEXTflex-96™ RNA-Seq Barcodes – 96 (in microfuge tubes)
768 rxns $3,077
 
 
   Benefits  
• Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads
• Input - 10 - 100 ng of mRNA or rRNA depleted total RNA
• Up to 96 barcodes available for multiplexing contain embedded index sequence
• Automation-friendly workflow is compatible with liquid handlers
• Functionally validated with GAIIx, HiSeq and MiSeq platforms

 
 
   Description  
The patent pending NEXTflex™ RNA-Seq Kits provide an easy and flexible solution for generating single, paired-end and multiplexed libraries from mRNA or rRNA depleted total RNA. Using the NEXTflex™ RNA-Seq Kits,  mRNA or rRNA depleted RNA is fragmented using a cationic buffer. Fragmented RNA undergoes first and second strand synthesis, followed by end-repair, adenylation, ligation and PCR.

Multiplexing
This kit was designed to be used in conjunction with the NEXTflex™ RNA-Seq or NEXTflex-96™ RNA-Seq Barcodes for multiplexing. The availability of up to 96 unique adapter barcodes makes this the most high-throughput kit available for RNA-Seq.
 
 
   Key Features  
The NEXTflex™ RNA-Seq Kit contains enough material to prepare 8 or 48 RNA samples for Illumina® compatible sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.
 
 
 
 
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  Related Product/Services
NEXTflex™ RNA-Seq Barcodes
NEXTflex-96™ RNA-Seq Barcodes
 

 
   Notes  
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
 

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