NEXTflex™ Directional RNA-Seq Kit (dUTP based) for Illumina sequencing

Products > Next-Generation Sequencing > Illumina-Compatible > RNA Sequencing

NEXTflex™ Directional RNA-Seq Kit (dUTP based)

for Illumina sequencing

Catalog#

Product Name

Quantity

US List Price

5129-05

NEXTflex™ Directional RNA-Seq Kit (dUTP-Based)

8 rxns

$457

5129-06

NEXTflex™ Directional RNA-Seq Kit (dUTP-Based)

48 rxns

$2,245

512911

NEXTflex™ RNA-Seq Barcodes – 6

48 rxns

$207

512912

NEXTflex™ RNA-Seq Barcodes – 12

96 rxns

$409

512913

NEXTflex™ RNA-Seq Barcodes – 24

192 rxns

$809

512914

NEXTflex™ RNA-Seq Barcodes - 48

384 rxns

$1,591

512915

NEXTflex-96™ RNA-Seq Barcodes – 96 (in 96-well plates)

768 rxns

$3,077

512916

NEXTflex-96™ RNA-Seq Barcodes – 96 (in microfuge tubes)

768 rxns

$3,077

Benefits

• dUTP-based (sense stranded) directional version of the NEXTflex RNA-Seq Kit
• Ideal for use when sequencing inserts > 150 bp
• Input - 10 - 100 ng of mRNA or rRNA depleted total RNA
• Up to 96 barcodes available for multiplexing contain embedded index sequence
• Automation-friendly workflow is compatible with liquid handlers
• Functionally validated with GAIIx, HiSeq and MiSeq platforms

Description

The patent pending NEXTflex™ Directional RNA-Seq Kit (dUTP-Based) provides an easy and flexible solution for generating single, paired-end and multiplexed libraries from mRNA or rRNA depleted total RNA. This kit is compatible with the NEXTflex™ RNA-Seq Barcodes and the NEXTflex-96™ RNA-Seq Barcodes.

This kit is ideal for directional sequencing of inserts that are > 150 bp. With smaller insert sizes we recommend using the ligation-based NEXTflex™ Directional RNA-Seq Kit (Ligation-Based).

Selected Reference:

Rossetto CC, Tarrant-Elorza M, Pari GS (2013) Cis and Trans Acting Factors Involved in Human Cytomegalovirus Experimental and Natural Latent Infection of CD14 (+) Monocytes and CD34 (+) Cells. PLoS Pathog 9(5): e1003366. doi:10.1371/journal.ppat.1003366

Key Features

The NEXTflex™ Directional RNA-Seq Kit (dUTP based) contains enough material to prepare 48 RNA samples for Illumina® compatible sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.

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