- Simplifies ELISA kits for end users - One-component, ready-to-use formulation
- Proven - Validated in hundreds of different types of ELISA
- Long shelf life – Stable for 3 years when stored at 2º - 8ºC
- Complete - No additional reagents or filtering required
- Safe - Non-carcinogenic with no DMF or DMSO present in the reagents
- Assured quality - ISO 9001:2008 certified
TMB is a substrate and chromogen that yields a blue color when oxidized, as a result of oxygen radicals produced by the reduction of hydrogen peroxide by HRP. For kinetic or non-stopped ELISA assays, the TMB chromogen has maximal absorbances at 370 nm and 652 nm. The color then changes to yellow with the addition of sulfuric or phosphoric acid, with maximum absorbance at 450 nm.
One-component, Ready-to-use TMB Formulation Simplifies ELISA Kits
The MaxSignal® TMB (3,3’,5,5’ – tetramethylbenzidine) Microwell Substrate Solution is a one-component, ready-to-use liquid formulation that requires no preparation before use. This simplifies ELISA kits for the end user while increasing accuracy by reducing potential errors. Unlike most other commercially available substrates, these products contain no DMF or DMSO.
TMB Validated in Hundreds of Different Types of ELISA Kits
The MaxSignal TMB Microwell Substrate Solution is a proven, robust solution that has been incorporated into hundreds of different types of ELISA kits developed for both the research and applied markets which have been sold throughout the world for many years. This substrate solution provides high signal and high sensitivity for ELISA kits.
TMB Offers a Long Shelf Life – Stable for 3 years at 2 - 8ºC
The MaxSignal TMB Microwell Substrate Solution has a shelf life of 3 years when stored at 2-8°C. This reagent has undergone rigorous stability testing to measure both its stability and performance over a prolonged period of time. Stability tests were conducted with the MaxSignal TMB Microwell Substrate Solution along with TMB obtained from other leading manufacturers. As seen in the figures below, the MaxSignal TMB Microwell Substrate Solution is more stable than competitors’ TMB when tested for 300 days at room temperature, 4°C and 37°C. This increased stability translates to increased kit stability for ELISA kit manufacturers.
This reagent is manufactured to the international quality standard ISO 9001:2008.
The MaxSignal TMB Microwell Substrate Solution can be stored at 2ºC – 8ºC for 3 years.