de Sousa et. al. from the University of Gothenburg and the Swedish Museum of Natural History recently developed 50 novel LCN markers in Medicago (which contains 87 species of annual and perennial herbs and shrubs, several of which are important global forage and green manure crops) and assessed the phylogenetic properties of each marker. They used the NEXTflex™ DNA-Seq Kit for library prep along with the the MYBaits target enrichment system to generate sequences. This protocol offered them a cost-effective approach to amplicon sequencing in phylogenetic studies at the genus or tribe level and allowed for an increase in number and size of targeted loci.
de Sousa, F., Bertrand, Y. J. K., Nylinder, S., Oxelman, B., Eriksson, J. S. and Pfeil, B. E. (2014) Phylogenetic Properties of 50 Nuclear Loci in Medicago (Leguminosae) Generated Using Multiplexed Sequence Capture and Next-Generation Sequencing. PLoS One. Doi 10.1371/journal.pone.0109704.