- Consistent, reproducible results
- High sensitivity – 50% B/Bo (< 1.0 ng/mL)
- Assay Range 0.1 ng/ml – 10 ng/ml
- Fast results – Less than 2 hours regardless of number of samples
The MaxSignal® Atropine ELISA Kit uses a competitive immunoassay method to determine the amount of atropine present in the blood or urine sample. Test plate wells are coated with atropine. Blood or urine sample is added for analysis, along with anti-atropine antibody. Atropine in the sample will compete for the primary antibody, thereby preventing the antibody from binding to the drug attached to the well. After incubation, the sample is removed, the wells are washed and a secondary HRP-conjugated antibody is added. The intensity of the absorbance at 450 nm is directly proportional to the amount of Atropine in the sample.
Atropine is an anticholinergic bronchodilator used as a performance-enhancing in racehorses. It is closely structurally related to ipratropium, another well-known bronchodilator in racehorses.
CAPACITY: 96 determinations or testing of 42 samples in duplicate
STORAGE CONDITIONS: 2-8°C
SHELF LIFE: 6 months when stored under proper conditions