- Consistent, reproducible results
- High sensitivity – 50% B/Bo (< 1.0 ng/mL)
- Assay Range 0.1 ng/ml – 10 ng/ml
- Fast results – Less than 2 hours regardless of number of samples
The MaxSignal® Beta/Dexamethasone ELISA Kit uses a competitive immunoassay method to determine the amount of dexamethasone, betamethasone and flumethasone present in a blood or urine sample. Test plate wells are coated with drug. Blood or urine sample is added for analysis, along with antibody against the glucocorticoids. Glucocorticoids in the sample will compete for the primary antibody, thereby preventing the antibody from binding to the drug attached to the well. After incubation, the sample is removed, the wells are washed and a secondary HRP-conjugated antibody is added. The intensity of the absorbance at 450 nm is directly proportional to the amount of drug in the sample.
Dexamethasone, Betamethasone and Flumethasone are glucocorticoids used for their anti-inflammatory properties. Glucocorticoids cause profound and varied metabolic effects and modify the body’s immune response to diverse stimuli.
CAPACITY: 96 determinations or testing of 42 samples in duplicate
STORAGE CONDITIONS: 2-8°C
SHELF LIFE: 6 months when stored under proper conditions