- Consistent, reproducible results
- High sensitivity – 50% B/Bo (< 1.0 ng/mL)
- Assay Range 0.1 ng/ml – 10 ng/ml
- Fast results – Less than 2 hours regardless of number of samples
The MaxSignal® Bupivacaine ELISA Kit uses a competitive immunoassay method to determine the amount of bupivacaine present in the blood or urine sample. Test plate wells are coated with bupivacaine. Blood or urine sample is added for analysis, along with anti-bupivacaine antibody. Bupivacaine in the sample will compete for the primary antibody, thereby preventing the antibody from binding to the drug attached to the well. After incubation, the sample is removed, the wells are washed and a secondary HRP-conjugated antibody is added. The intensity of the absorbance at 450 nm is directly proportional to the amount of Bupivacaine in the sample.
Bupivacaine is a local anaesthetic drug which blocks the conduction of nerve fibers. Equine administration is detectible for up to 20-24 hours post-administration.
CAPACITY: 96 determinations or testing of 42 samples in duplicate
STORAGE CONDITIONS: 2-8°C
SHELF LIFE: 6 months when stored under proper conditions