- Consistent, reproducible results
- High sensitivity – 50% B/Bo (< 1.0 ng/mL)
- Assay Range 0.1 ng/mL – 10 ng/mL
- Fast results – Less than 2 hours regardless of number of samples
The MaxSignal® Methadone ELISA Kit uses a competitive immunoassay method to determine the amount of methadone present in a serum or urine sample. Test plate wells are coated with methadone. Blood or urine sample is added for analysis, along with antibody against methadone. Methadone in the sample will compete for the primary antibody, thereby preventing the antibody from binding to the drug attached to the well. After incubation, the sample is removed, the wells are washed and a secondary HRP-conjugated antibody is added. The intensity of the absorbance at 450 nm is directly proportional to the amount of drug in the sample.
Methadone is a synthetic opioid used to treat pain, as well as to control withdrawal symptoms of narcotic drug addiction.
CAPACITY: 96 determinations or testing of 42 samples in duplicate
STORAGE CONDITIONS: 2-8°C
SHELF LIFE: 6 months when stored under proper conditions