- Consistent, reproducible results
- High sensitivity – 50% B/Bo (< 1.0 ng/mL)
- Assay Range 0.1 ng/mL – 10 ng/mL
- Fast results – Less than 2 hours regardless of number of samples
The MaxSignal® Phenytoin ELISA Kit uses a competitive immunoassay method to determine the amount of phenytoin present in a serum or urine sample. Test plate wells are coated with phenytoin. Blood or urine sample is added for analysis, along with antibody against phenytoin. Phenytoin in the sample will compete for the primary antibody, thereby preventing the antibody from binding to the drug attached to the well. After incubation, the sample is removed, the wells are washed and a secondary HRP-conjugated antibody is added. The intensity of the absorbance at 450 nm is directly proportional to the amount of drug in the sample.
Phenytoin is an antiepileptic drug. The site of action is believed to be the motor cortex, where spread of seizure activity is inhibited. Phenytoin is believed to have a tranquilizing affect in animals.
CAPACITY: 96 determinations or testing of 42 samples in duplicate
STORAGE CONDITIONS: 2-8°C
SHELF LIFE: 6 months when stored under proper conditions