- Consistent, reproducible results
- High sensitivity 50% B/Bo (0.10 ng/mL)
- Assay Range 0.05 ng/mL – 10.0 ng/mL
- Fast results - less than 2 hours regardless of number of samples
The MaxSignal® Sildenafil/Vardenafil ELISA Kit uses a competitive immunoassay method to determine the amount of sildenafil or vardenafil present in a serum or urine sample. Test plate wells are coated with drug. Serum or urine sample is added for analysis, along with antibody against the drug. Sildenafil or vardenafil in the sample will compete for the primary antibody, thereby preventing the antibody from binding to the drug attached to the well. After incubation, the sample is removed, the wells are washed and a secondary HRP-conjugated antibody is added. The intensity of the absorbance at 450 nm is directly proportional to the amount of drug in the sample.
Sildenafil was originally developed as an agent to lower blood pressure. Both Sildenafil and Vardenafil are widely used in impotence therapy.
CAPACITY: 96 determinations or testing of 42 samples in duplicate
STORAGE CONDITIONS: 2-8°C
SHELF LIFE: 6 months when stored under proper conditions