- The only commercially available ELISA for clonidine detection
- High recovery rates and rapid cost-effective extraction methods
- High sensitivity - 0.015 ppb
- Fast ELISA assay which takes less than 2 hours regardless of number of samples
- High reproducibility
The MaxSignal® Clonidine ELISA Test Kit is the only commercially available ELISA for the rapid screening of clonidine in fish, shrimp, meat, feed and urine samples.
As one of ß-agonists, clonidine can be to increase muscle growth and appetite in livestock. Although steroids result in a higher meat/fat ratio, there is the risk that residues will be left in meat products and which are harmful when consumed to the consumer. The use of clonidine in food production is forbidden in many countries.
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
This kit is manufactured to the international quality standard ISO 9001:2008.
The MaxSignal Clonidine ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.