- Complete, ready-to-use kit
- Cost effective
- Quantitative determination of protein concentration
- Sensitive - detect as little as 15 pg/mL
- Reliable - thoroughly tested gene-specific kit
- Easy to use- standards are pre-calibrated
- Colorimetric detection
The MaxDiscovery™ Human G-CSF ELISA Test Kit is designed for quantitative determination of the concentration of human G-CSF in serum, plasma, and cell culture supernatant.
Human granulocyte colony stimulating factor (G-CSF) is an O-glycosylated 19.6 kDa glycoprotein which is secreted by monocytes, macrophages and neutrophils after cell activation. G-CSF is produced also by stromal cells, fibroblasts, and endothelial cells. It stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophil/granulocyte lineage in a dose-dependent manner. A combination of IL-4 with G-CSF has been shown to lead to synergistic suppression of the growth of some human leukemic cell lines. The main and most important clinical application of G-CSF is probably the treatment of transient phases of leukopenia following chemotherapy and/or radiotherapy.
Format: 96-well strip plate
Assay range: 15-960 pg/mL
Sensitivity: 15 pg/mL
Total Assay Time: 3 hours and 45 minutes
Sample Size: 100 µL/well
Sample types: Serum, Plasma and Cell Culture Supernatant
Wavelength for plate reading: 450 nm