- Complete, ready-to-use kit
- Cost effective
- Quantitative determination of protein concentration
- Sensitive - detect as little as 8 pg/mL
- Reliable - thoroughly tested gene-specific kit
- Easy to use- standards are pre-calibrated
- Colorimetric detection
The MaxDiscovery™ Human IL-10 ELISA Test Kit is designed for quantitative determination of the concentration of human IL-10 in serum, plasma, and cell culture supernatant.
Human interleukin 10 (IL-10) is a homodimeric protein with subunits having a length of 160 amino acids. It shows 73 percent amino acid homology with murine IL-10. It is produced by activated CD8 + peripheral blood T cells, by T-helper CD4 + T cell clones after both antigen-specific and polyclonal activation, by B-cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells. IL-10 inhibits the synthesis of a number of cytokines such as IL-2 and TNF-β in Th1 T-helper cells but not of Th2 T-helper cells. IL-10 also inhibits mitogen or anti-CD3 induced proliferation of T cells in the presence of accessory cells and reduces the production of IFN-γ and IL-2.
Format: 96-well strip plate
Assay range: 8-512 pg/mL
Sensitivity: 8 pg/mL
Total Assay Time: 3 hours and 45 minutes
Sample Size: 100 µL/well
Sample types: Serum, Plasma and Cell Culture Supernatant
Wavelength for plate reading: 450 nm