- Complete, ready-to-use kit
- Cost effective
- Quantitative determination of protein concentration
- Sensitive - detect as little as 8 pg/mL
- Reliable - thoroughly tested gene-specific kit
- Colorimetric detection
The MaxDiscovery™ Human IL-5 ELISA Test Kit is designed for quantitative determination of the concentration of human IL-5 in serum, plasma, and cell culture supernatant.
Human interleukin-5 (IL-5) is a ~13 kDa polypeptide of 115 amino acids after cleavage of a signal peptide. It is mainly produced by T cells in response to stimulation by parasite-derived antigens and allergens. IL-5 is a specific hematopoietic growth factor responsible for the growth and differentiation of eosinophils. It promotes the growth of immature hematopoietic progenitor cells. IL-5 strongly stimulates the proliferation, cell activation, and differentiation of eosinophilic granulocytes. IL-5 also promotes the generation of cytotoxic T-cells from thymocytes. Some asthmatic patients and cancer patients using IL-2 immunotherapy showed elevated levels of serum IL-5.
Format: 96-well strip plate
Assay range: 8-512 pg/mL
Sensitivity: 8 pg/mL
Total Assay Time: 3 hours and 45 minutes
Sample Size: 100 µL/well
Sample types: Serum, Plasma and Cell Culture Supernatant
Wavelength for plate reading: 450 nm