- Complete, ready-to-use kit
- Cost effective
- Quantitative determination of protein concentration
- Sensitive - detect as little as 10 pg/mL
- Reliable - thoroughly tested gene-specific kit
- Colorimetric detection
The MaxDiscovery™ Human IL-6 ELISA Test Kit is designed for quantitative determination of the concentration of human interleukin 6 (IL-6) in serum, plasma, and cell culture supernatant.
IL-6 is a 20.5 kDa protein containing 184 amino acid residues. It is a multifunctional protein that plays important roles in host defense, acute phase reactions, immune responses, and hemotopoiesis. Synonyms for IL-6 include: B cell stimulatory factor-2 (BSF-2), hybridoma/plasmacytoma growth factor, hepatocyte stimulating factor, cytotoxic T cell differentiation factor and macrophage-granulocyte inducing factor 2A (MGI-2A). IL-6 is expressed by a variety of normal and transformed cells including T cells, B cells, monocytes/macrophages, carcinomas, sarcomas, and many more. In comparison with mouse IL-6, human IL-6 exhibits approximately 65% sequence homology at the nucleotide level, and 42% homology at the amino acid level. In contrast to its growth stimulatory actitivities, IL-6 is also a growth inhibitor for a number of leukemia and carcinoma cells lines.
Na, J. O. et al. (Dec, 2010) Impaired transport function of the left atrium and left atrial appendage in cryptogenic stroke patients with atrial septal aneurysm and without patent foramen ovale Eur J Echocardiogr 12 (2): 140-147. doi: 10.1093/ejechocard/jeq164
Tsui, K-H et al. (July, 2010) Mechanisms by which Interleukin-6 Regulates Prostate Specific Antigen Gene Expression in Prostate LNCaP Carcinoma Cells. J Andrology, 32: 383 - 393. doi: 10.2164/jandrol.109.009878.
Shin, S. Y. et al., (April, 2011) Improved Endothelial Function in Patients with Atrial Fibrillation through Maintenance of Sinus Rhythm by Successful Catheter Ablation. J Cardiovasc Electr. 22 (4): 376 – 382.
Format: 96-well strip plate
Assay range: 10-640 pg/mL
Sensitivity: 10 pg/mL
Total Assay Time: 3 hours and 45 minutes
Sample Size: 100 µL/well
Sample types: Serum, Plasma and Cell Culture Supernatant
Wavelength for plate reading: 450 nm