- Complete, ready-to-use kit
- Cost effective
- Quantitative determination of protein concentration
- Sensitive - detect as little as 16 pg/mL
- Reliable - thoroughly tested gene-specific kit
- Colorimetric detection
The MaxDiscovery™ Human IL-8 ELISA Test Kit is designed for quantitative determination of the concentration of human interleukin 8 (IL-8) in serum, plasma, and cell culture supernatant.
Human IL-8 is an 8 kD peptide consisting of 77 amino acid residues after cleavage of 22 residue signal peptide. Further proteolysis of the N-terminal end generates a 72 amino acid peptide with full biological activity. As a member of the neutrophil-specific CXC subfamily of chemokines, IL-8 is a potent neutrophil chemtactic and activating factor. In response to proinflammatory stimuli such as IL-1, TNF, LPS and virus, IL-8 is produced by monocytes/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, astrocytes and chondrocytes. The function of IL-8 is to attract neutrophils to the site of inflammation and to activate them. IL-8 binds to two G protein-coupled receptors, CXCR1 and CXCR2, as well as to the Duffy antigen on red blood cells.
Format: 96-well strip plate
Assay range: 16-1024 pg/mL
Sensitivity: 16 pg/mL
Total Assay Time: 3 hours and 45 minutes
Sample Size: 100 uL/well
Sample types: Serum, plasma and Cell Culture Supernatant
Wavelength for plate reading: A450 nm