- Complete, ready-to-use kit
- Cost effective
- Quantitative determination of protein concentration
- Sensitive - detect as little as 8 pg/mL
- Reliable - thoroughly tested gene-specific kit
- Colorimetric detection
The MaxDiscovery™ Human TNF-α ELISA Test Kit is designed for quantitative determination of the concentration of human tumor necrosis factor alpha (TNF-α, also known as cachectin) in serum, plasma, and cell culture supernatant.
Human TNF-α is a 17.5 kDa polypeptide of 157 amino acids. It is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells. This cytokine is a primary regulator of inflammatory responses. TNF-α is produced by macrophages, neutrophils, activated T and B lymphocytes, endothelial cells, smooth muscle cells, astrocytes, natural killer cells, lymphokine-activated killer cells, and some transformed cells. TNF-α, and LT-α (lymphotoxin cellular receptors (CD 120a and CD120b), and produce similar effects. TNF-α plays a critical role in the destruction of certain tumors and in the body’s resistance to infection by inducing fever and activating macrophages. Over-production of TNF has been associated with cytotoxic effects such as cachexia.
Grigorie D, Sucaliuc A et al. (2013) Circulating RANKL is Not a Reliable Biomarker for Bone Loss in Primary Hyperparathyroidism. ROM. J. INTERN. MED., 2013, 51, 1, 23–28
Shin, S. Y. et al., (April 2011) Total and Interatrial Epicardial Adipose Tissues Are Independently Associated With Left Atrial Remodeling in Patients With Atrial Fibrillation. J Cardiovasc Electr. 22 (6): 647 – 655.
Format: 96-well strip plate
Assay range: 8-512 pg/mL
Sensitivity: 8 pg/mL
Total Assay Time: 3 hour and 45 minutes
Sample Size: 100 µL/well
Sample types: Serum, Plasma and Cell Culture Supernatant
Wavelength for plate reading: 450 nm