Amplicon Panels for Illumina Platforms

NEXTflex® Amplicon Panel Kits


Complete kits including target-specific primers, library prep reagents and barcodes for Illumina® sequencing

Amplicon sequencing is based on ultra-deep sequencing of PCR products for analyzing genetic variations and differences in gene expression. Validated custom and pre-designed amplicon-based assays allow researchers to focus interrogation on key regions of genomic interest using a simple workflow.

The NEXTflex® Amplicon Panels allow researchers to sequence large numbers of targeted gene regions from selected samples for analysis of mutational hot spots within a subset of genes or detection of copy number variations (CNVs), well-defined gene fusions, SNPs or Indels. The NEXTflex Amplicon Panels are easily scalable, simple to use, fast, cost-effective kits which can be applied to a broad range of organisms and/or genes.

The NEXTflex Amplicon Panels include primers flanking the regions of interest, library prep reagents, clean-up beads and barcodes needed to construct libraries compatible with Illumina® sequencing platforms.

For research use only. Not for use in diagnostic procedures.


NEXTflex® Amplicon Panels for use with Genomic DNA Genes Covered
 Autism Spectrum Disorders PDE8B, EN2, NLGN4X, CDKL5, NLGN3, MECP2, RPL10
BRCA1/2 XP BRCA1, BRCA2
BRCA1/2 Plus-1 BRCA1, BRCA2, PALB2, CHEK2
CEBPA CEBPA
Colorectal Cancer -1 MLH1, MSH2
Colorectal Cancer-2 MSH6, PMS2
Congenital Adrenal Hyperplasia CYP21A2
Congenital Hyperinsulism ABCC8, GLUD1, KCNJ11, GCK, HADH, HNF4A, INS, INSR, PDX1, SLC16A1 & UCP2
CVD 22 hot spots for cardiovascular disease: MTHFR, F5, AGT, APOB, AGTR1, FGB, F13A1, LTA, SERPINE1, NOS3, JAK2, F2, ITGB3, APOE, PROCR, CBS
Cystic Fibrosis CFTR
Duchenne Muscular Dystrophy DMD
Epilepsy-2 SCN2A, SCN9A
Female Infertility FSHB, FSHR, LHB, LHCGR
HBOC-1 RAD51D, RAD51C, BRIP1
HBOC-2 PALB2, BARD1, TP53
Lysosomal Storage Diseases SUMF1, GLB1, IDUA, ARSB, GUSB, SMPD1, GALC, GALNS, GAA, GLA, IDS
Male Infertility AR, CATSPER1, CFTR, FSHR, LHCGR
Marfan Syndrome FBN1
Mediterranean Fever MEFV
MODY-1 HNF1A, GCK
MODY-2 HNF1B, HNF4A
MODY-3 PDX1, NEUROD1, KLF11, CEL
MODY-4 PAX4, INS, BLK
MODY-5 GCK, HNF1A, HNF1B, HNF4A
Myeloid Selected CDS from 21 genes 
Nephrotic Syndrome-1 NPSH1, NPSH2, WT1
Nephrotic Syndrome-2 ARHGDIA, DGKE, LAMB2, PLCE1
Neurofibromatosis NF1, NF2
Neuronal Ceroid Lipofuscinoses CLN3, CLN5, CLN6, CLN8, CTSD, MFSD8, PPT1, TPP1
Obesity-1 LEP, LEPR, POMC, MC4R
Obesity-2 KSR2, SH2B1, SIM1
Periodic Fever-1 TNFRSF1A, NLRP3, MVK
Periodic Fever-2 ELANE, LPIN2, PSTPIP1
Phenylketonuria PAH
TP53 TP53
NEXTflex® Amplicon Panels for Use with DNA Isolated from FFPE Samples Genes Covered
BRCA FFPE BRCA1, BRCA2
TP53 FFPE TP53


The purchase price of this Product includes a limited, non-transferable license under U.S. and foreign patents owned by BIO-RAD Laboratories, Inc., to use this Product. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this Product. Phusion DNA Polymerase is manufactured by Thermo Fisher Scientific. PhusionTM is trademark or registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries.