Amplicon Panels for Illumina Platforms

NEXTflex® Amplicon Panel Kits


Complete kits including target-specific primers, library prep reagents and barcodes for Illumina® sequencing

Amplicon sequencing is based on ultra-deep sequencing of PCR products for analyzing genetic variations and differences in gene expression. Validated custom and pre-designed amplicon-based assays allow researchers to focus interrogation on key regions of genomic interest using a simple workflow.

The NEXTflex® Amplicon Panels allow researchers to sequence large numbers of targeted gene regions from selected samples for analysis of mutational hot spots within a subset of genes or detection of copy number variations (CNVs), well-defined gene fusions, SNPs or Indels. The NEXTflex Amplicon Panels are easily scalable, simple to use, fast, cost-effective kits which can be applied to a broad range of organisms and/or genes.

The NEXTflex Amplicon Panels include primers flanking the regions of interest, library prep reagents, clean-up beads and barcodes needed to construct libraries compatible with Illumina® sequencing platforms.

For research use only. Not for use in diagnostic procedures.


NEXTflex® Amplicon Panels for use with Genomic DNA Genes Covered
 Autism Spectrum Disorders PDE8B, EN2, NLGN4X, CDKL5, NLGN3, MECP2, RPL10
BRCA1/2 BRCA1, BRCA2
BRCA1/2 Plus-1 BRCA1, BRCA2, PALB2, CHEK2
CEBPA CEBPA
Colorectal Cancer -1 MLH1, MSH2
Colorectal Cancer-2 MSH6, PMS2
Congenital Adrenal Hyperplasia CYP21A2
Congenital Hyperinsulism ABCC8, GLUD1, KCNJ11, GCK, HADH, HNF4A, INS, INSR, PDX1, SLC16A1 & UCP2
CVD 22 hot spots for cardiovascular disease: MTHFR, F5, AGT, APOB, AGTR1, FGB, F13A1, LTA, SERPINE1, NOS3, JAK2, F2, ITGB3, APOE, PROCR, CBS
Cystic Fibrosis CFTR
Duchenne Muscular Dystrophy DMD
Epilepsy-2 SCN2A, SCN9A
Female Infertility FSHB, FSHR, LHB, LHCGR
HBOC-1 RAD51D, RAD51C, BRIP1
HBOC-2 PALB2, BARD1, TP53
Lysosomal Storage Diseases SUMF1, GLB1, IDUA, ARSB, GUSB, SMPD1, GALC, GALNS, GAA, GLA, IDS
Male Infertility AR, CATSPER1, CFTR, FSHR, LHCGR
Marfan Syndrome FBN1
Mediterranean Fever MEFV
MODY-1 HNF1A, GCK
MODY-2 HNF1B, HNF4A
MODY-3 PDX1, NEUROD1, KLF11, CEL
MODY-4 PAX4, INS, BLK
MODY-5 GCK, HNF1A, HNF1B, HNF4A
Myeloid Selected CDS from 21 genes 
Nephrotic Syndrome-1 NPSH1, NPSH2, WT1
Nephrotic Syndrome-2 ARHGDIA, DGKE, LAMB2, PLCE1
Neurofibromatosis NF1, NF2
Neuronal Ceroid Lipofuscinoses CLN3, CLN5, CLN6, CLN8, CTSD, MFSD8, PPT1, TPP1
Obesity-1 LEP, LEPR, POMC, MC4R
Obesity-2 KSR2, SH2B1, SIM1
Periodic Fever-1 TNFRSF1A, NLRP3, MVK
Periodic Fever-2 ELANE, LPIN2, PSTPIP1
Phenylketonuria PAH
TP53 TP53
NEXTflex® Amplicon Panels for Use with DNA Isolated from FFPE Samples Genes Covered
BRCA FFPE BRCA1, BRCA2
TP53 FFPE TP53