- Facilitates the screening of a variety of food samples for BaP contamination
- Offers a high recovery rates
- Rapid, and cost-effective extraction methods
- High sensitivity 0.25 ppb
- Quick ELISA assay less than 2 hours regardless of the number of samples
- High reproducibility
MaxSignal® Benzo[a]pyrene (BaP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Benzo[a]pyrene (BaP) in a variety of sample types. Petroleum oils contain compounds that can be considered hazardous to human health, specifically polycyclic aromatic hydrocarbons (PAH). Studies have shown several of these PAHs in petroleum oil to be probable human carcinogens, or cancer causing agents, from lifetime exposure studies. It is this cancer risk that is the potential health risk.
The method is based on a competitive colorimetric ELISA assay. The BaP conjugate has been coated in the plate wells. During the analysis, a sample is added along with the primary antibody specific for BaP. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the BaP attached to the wells. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody associated with the BaP coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
This kit is manufactured to the international quality standard ISO 9001:2008.
The MaxSignal Benzo[a]pyrene (BaP) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C.