- Rapid extraction methods with high recovery
- High sensitivity (0.015 ppb) and low detection limit (0.1 ppb for meat, 0.1 ppb for urine sample)
- A quick ELISA assay - less than 2 hours regardless of number of samples
- High reproducibility
The MaxSignal® Cyproheptadine ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of cyproheptadine in meat, liver, kidney and urine.
The MaxSignal Cyproheptadine ELISA Test Kit is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Cyproheptadine, also known as as periactin, is a first-generation antihistamine with anticholinergic, antiserotonergic and local anesthetic properties. Cyproheptadine is used as a veterinary drug. Possible adverse effects include excitement and aggressive behavior. The amount of cyproheptadine residues can be determined by methods such as GC-MS or LC-MS-MS. However, these traditional methods usually require very expensive preparation procedures. Alternatively, enzyme immunoassay (ELISA) can be used as a screening system, which is simple, rapid, sensitive and cost-effective compared with traditional methods.
This kit is manufactured to the international quality standard ISO 9001:2008.
The MaxSignal Cyproheptadine ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.