MaxSignal® Histamine Enzymatic Assay Kit

 

Catalog# Product Name Quantity US List Price
1032-04 MaxSignal® Histamine Enzymatic Assay Kit 1 x 96 wells Inquire
1032-05 MaxSignal® Histamine Enzymatic Assay Kit 1 x 96 wells Inquire

These kits are available for purchase by email, fax or phone only. Please email your order to sales@biooscientific.com, fax it to 1-512-707-8122 or call 512-707-8993.

 

 

  • Simple - add sample and master mix to plate
  • Sensitive histamine detection - 4 ppm detection limit
  • Faster results – as little as 15 minutes from sample to results
  • Saves money - No expensive instrumentation required
  • High recovery rates

 


Sensitive ELISA for Histamine Detection in Fish, Seafood and Cheese

The MaxSignal® Histamine Enzymatic Assay Kit is a colorimetric enzymatic assay for the determination of histamine in fresh fish, seafood and cheese. The entire assay can be completed in less than 15 minutes.  Cat # 1032-04 uses a simple heating extraction method with no organic solvents while Cat # 1032-05 kit uses methanol-based extraction procedure which does not require heating.

Histamine is a contaminant sometimes found in seafood when improperly handled. High levels of histamine in seafood can cause scombroid poisoning. Quality tuna has histamine levels below 50 ppm. The amount of histamine can be determined using ELISA or LC-MS. However these traditional methods require very expensive instrumentation and time consuming sample preparation procedures. 

Watch the MaxSignal Histamine Enzymatic Assay Kit Demonstration


Selected Publications that Reference the MaxSignal Histamine ELISA: 

Hall, G. (2010) Fish Processing: Sustainability and New Opportunities. John Wiley and Sons. pg 163. 

 

Hungerford, J. M. (August 15, 2010) Scombroid Poisoning: A Review. Toxicon. 56:2, pg 231-243. http://dx.doi.org/10.1016/j.toxicon.2010.02.006 

You can also download the HACCP planning document for histamine testing entitled “HACCP Advice for Producers of Scombroid Species Fish Products” here. 

This kit is manufactured to the international quality standard ISO 9001:2008.


Kit Specs

PLATE: 1 x 96 well (Removable 8 wells x 12 strips) 
MEASUREMENT: microtiter plate reader (450 nm)
TESTS PER KIT: The kit has the capacity for 96 determinations or testing of 42 samples in duplicate 
STANDARDS: 0 / 0.35 / 0.7 / 1.4 / 2.8 / 7 ppm
INCUBATION TIME: 10 minutes 
SHELF LIFE & STORAGE: 12 months when properly stored

Recovery of Histamine from Yellowfin Tuna

Histamine Analysis of Yellowfin Tuna

 

Recovery of Histamine from Albacore Tuna

Histamine Analysis of Albacore Tuna

 

Recovery of Histamine Spikes

Increased Histamine Recovery Rates