- Rapid, cost-effective extraction methods offering high recovery rates
- High sensitivity - 5.0 ppb
- Fast assay requiring less than 1.5 hours regardless of the number of samples
- High reproducibility
Sensitive Detection of Pirlimycin in Milk
The MaxSignal® Pirlimycin ELISA Test Kit offers a quantitative analysis of pirlimycin in milk. This kit is a competitive colorimetric ELISA assay. Anti-pirlimycin antibody has been coated in the plate wells. During analysis, sample is added along with the pirlimycin-horseradish peroxidase (Pirlimycin-HRP) conjugate. If pirlimycin residue is present in the sample, it will compete for the pirlimycin antibody, thereby preventing the Pirlimycin-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the pirlimycin residue concentration in the sample.
Pirlimycin is a lincosamide antibiotic. Under the trade name Pirsue, it is used to treat mastitis in cattle. Pirlimycin is active against gram-positive bacteria, specifically Staphylococcus aureus and coagulase-negative species of Staphylococcus and Streptococcus. It is bacteriostatic and acts by inhibiting bacterial protein synthesis via binding with the 50S subunit of the ribosome.
An MRL for Pirlimycin of 100 ppb in milk is approved in the European Union and New Zealand; 400 ppb is approved in both the United States and in Canada, and Japan recently recommended a milk MRL of 300 ppb.
This kit is manufactured to the international quality standard ISO 9001:2008. ISO CI#: SARA-2009-CA-0114-A
The MaxSignal Pirlimycin ELISA Test Kit has the capacity for 96 determinations, or testing of 42 samples in duplicate (assuming 12 wells for standards). Most of the kit components should be stored at 2-8°C, except for the Pirlimycin Standards, which should be stored at -20°C (or 4°C for up to 1 week). The shelf life is 12 months when the kit is properly stored.