- High recovery rates are obtained using low-cost, rapid (~ 1 hour) sample preparation protocols
- High sensitivity: 10 ppb for honey and milk samples
- Rapid assay protocol (less than 50 minutes to process 42 samples in duplicate)
- High reproducibility (interplate and intraplate CV typically < 10%)
Sensitive Spiramycin Detection in Honey and Milk
The MaxSignal® Spiramycin ELISA Test Kit is a rapid ELISA for the quantitative analysis of spiramycin in honey, milk, and meat samples. Spiramycin (Rovamycine) is a 16-membered ring macrolide. It is used to treat toxoplasmosis and various other infections of soft tissues and as a feed additive to improve feed transformation efficiency and increase body weight.
The MaxSignal Spiramycin ELISA Test Kit is a competitive colorimetric ELISA assay. The plate wells are coated with anti-spiramycin antibody. During analysis, sample is added along with the Spiramycin-horseradish peroxidase (Spiramycin-HRP) conjugate. If spiramycin residue is present in the sample, it will compete for the spiramycin antibody, thereby preventing the Spiramycin-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the concentration of spiramycin residue in the sample.
The MaxSignal Spiramycin ELISA Test Kit offers significant cost and time savings over high pressure liquid chromatography which is widely used for spiramycin detection.
This kit is manufactured to the international quality standard ISO 9001:2008. ISO CI#: SARA-2009-CA-0114-A.
The MaxSignal Spiramycin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.