- Complete, ready-to-use kit
- Cost effective
- Quantitative determination of protein concentration
- Sensitive - Detect as little as 10 pg/mL
- Reliable - Thoroughly tested gene-specific kit
- Colorimetric detection
The MaxDiscovery™ Mouse IFN-γ ELISA Test Kit is designed for quantitative determination of the concentration of mouse IFN-γ in serum, plasma, and cell culture supernatant.
Mouse interferon gamma (IFN-γ), also called Type II interferon, macrophage activation factor, and immune interferon, is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS polyarylamide gel it appears as a combination of 25, 20 and minor 15.5 kDa bands due to differential glycosylation. IFN-γ is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. The biological activity of the IFN-γ homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, enhances immunoglobulin secretion by B lymphocytes, acts in cytokine regulation and also acts synergistically with other cytokines.
Publications that Cite Using the MaxDiscovery™ Mouse IFN-γ ELISA Test Kit
Gohar, A. Immune therapy against diarrheagenic Escherichia coli. Cairo University, 2016.
Format: 96-well strip plate
Assay range: 10-640 pg/mL
Sensitivity: 10 pg/mL
Total Assay Incubation Time: 3 hours and 45 minutes
Sample Size: 100 µL/well
Sample types: Serum, Plasma and Cell Culture Supernatant
Wavelength for plate reading: 450 nm